A new and rapid determination of pyridinium aldoximes in blood and urine.

A rapid and accurate method for analyzing pyridinium oximes-N-methylpyridinium2-aldoxime chloride, N,N’-trimethylene-(pyridinium-4-aldoxime) dihalide, and N,N’oxydimethyl(pyridinium-4-aldoxime) dich bride-in plasma, urine, and whole blood is described.The method is completely automated and requires small sample volumes. Concentrations ranging from 3 to 120 Eq./L. in biologic fluids can be determined at a rate of 40 samples per hour. This technic can be applied to oximes which are unstable in basic solution. The average variation of the oxime concentration usedto establish calibration curves, as determined by the ratio of the standard deviation to the mean, was ± 1.5%. Plasma and albumin increase the transfer rate of the oximes through the dialyzing membrane. Theoretical concentrations to explain this phenomenonare presented.